Study of Post-transcriptional Gene Regulation with Interdisciplinary Approaches
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https://www.dnalc.org/resources/3d/rna-splicing.html
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Post-transcriptional regulation plays an indispensable role in fine-tuning the accuracy of gene expression. The information encoded in RNA directs the outcome of splicing, transportation, stability and translation efficiency. I am interested in studying how the primary sequence of RNA determines its fate of expression. We will combine computational analysis and biochemical approaches to study the post-transcriptional regulation in a genome-wide fashion.
Pre-mRNA splicing:
Alternative pre-mRNA splicing is the key post-transcriptional regulation step by which the same DNA sequence can be expressed into various different isoforms. The splice sites are recognized by the small RNA in the gigantic splicing machinery, spliceosome. In the first step of splicing, 5' splice site (5'ss) interacts with U1 small nuclear ribonucleoprotein (snRNP) complex in the spliceosome, and the branchpoint sequence interacts with the U2 snRNP, which then helps determine the 3' splice site (3'ss). The branchpoint sequence recognition is regulated differently in different cell types and developmental stages. I am interested in knowing how RNA-binding proteins can help the recognition and transition into different branchpoint usage during the course of developement and differentiation.
mRNA translation:
Local mRNA translation is important for responding to stimulus locally in a timely fashion. 3' untranlated region (3'UTR) of mRNA contains sequence elements with affinity to RNA-binding proteins and small RNAs that control its stability, localization and translation. I am interested in studying how natural mutations and sequence polymorphisms in the 3'UTR could affect phenotypes (such as diseases) through the post-transcriptional regulation.
本實驗室以基因體學與分子生物學的角度研究轉錄後的基因調控，包括RNA的剪接、穩定性與轉譯。主要以人類細胞株，利用資訊生物學、生化、分生與次世代定序等等的方法研究基因序列在轉錄後調控所扮演的腳色。
Check out the Video involving my postdoc research : Can RNA Splicing Errors Cause Disease?
Pre-mRNA splicing:
Alternative pre-mRNA splicing is the key post-transcriptional regulation step by which the same DNA sequence can be expressed into various different isoforms. The splice sites are recognized by the small RNA in the gigantic splicing machinery, spliceosome. In the first step of splicing, 5' splice site (5'ss) interacts with U1 small nuclear ribonucleoprotein (snRNP) complex in the spliceosome, and the branchpoint sequence interacts with the U2 snRNP, which then helps determine the 3' splice site (3'ss). The branchpoint sequence recognition is regulated differently in different cell types and developmental stages. I am interested in knowing how RNA-binding proteins can help the recognition and transition into different branchpoint usage during the course of developement and differentiation.
mRNA translation:
Local mRNA translation is important for responding to stimulus locally in a timely fashion. 3' untranlated region (3'UTR) of mRNA contains sequence elements with affinity to RNA-binding proteins and small RNAs that control its stability, localization and translation. I am interested in studying how natural mutations and sequence polymorphisms in the 3'UTR could affect phenotypes (such as diseases) through the post-transcriptional regulation.
本實驗室以基因體學與分子生物學的角度研究轉錄後的基因調控，包括RNA的剪接、穩定性與轉譯。主要以人類細胞株，利用資訊生物學、生化、分生與次世代定序等等的方法研究基因序列在轉錄後調控所扮演的腳色。
Check out the Video involving my postdoc research : Can RNA Splicing Errors Cause Disease?
THE TEAM
Chien-Ling Lin, Ph.D.
Principle Investigator CV
EDUCATION University of Massachusetts Medical School, Worcester, MA, USA 2005-2012 Doctor of Philosophy, Program in Molecular Medicine University College London, London, United Kingdom 2002-2003 Master of Science, Neuroscience National Taiwan University, Taipei, Taiwan 1998-2002 Bachelor of Science, Zoology SCIENTIFIC POSITIONS Assistant Research Fellow 2017- Institute of Molecular Biology, Academia Sinica, Taiwan Visiting Scholoar 2016- 2017 Brown University, Providence, RI Postdoctoral Research Associate 2012-2016 Brown University, Providence, RI Research Assistant 2005-2012 University of Massachusetts Medical School, Worcester, MA Research Associate 2003-2005 Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan Postdocs and Research Assistants Wanted:
招募具備分生、生化或生物資訊等背景及經驗，對跨領域研究有興趣者。 |
News> 2026 Jan
恭喜江宏倫博士 獲得中央研究院分子生物研究所博士後研究學者合作計畫獎助! Congratulations to Dr. Hung-Lun Chiang on the Institute of Molecular Biology Postdoctoral Scholar Program > 2026 Jan 恭喜蘇家瑩博士 獲得中央研究院博士後研究學者獎助! Congratulations to Dr. Jia-Ying Su on Postdoctoral Scholar Fellowship, Academia Sinica > 2025 Oct 恭喜蘇家瑩博士於台灣RNA研討會壁報比賽榮獲優勝! Congratulations to Dr. Jia-Ying Su on the Poster Merit Award of the 2025 Taiwan RNA Symposium! 
> 2025 Jan
恭喜江宏倫博士 獲得中央研究院分子生物研究所博士後研究學者合作計畫獎助! Congratulations to Dr. Hung-Lun Chiang on the Institute of Molecular Biology Postdoctoral Scholar Program >2024 July 恭喜雲俊源獲得國科會大專學生研究計畫! >2023Dec 賀!!! 恭喜GSB博班生黃昂矩榮獲罕見疾病基金會罕見疾病博碩士論文獎助! https://www.tfrd.org.tw/tfrd/news/content/id/3324 > 2023Dec 恭喜林倩伶老師獲選為歐洲分子生物學組織「全球研究學者」 Dr. Chien-Ling Lin has been selected to join the EMBO Global Investigator Network. https://www.embo.org/press-releases/embo-announces-new-global-investigators/ newsletter.sinica.edu.tw/ > 2023Nov 恭喜GSB博班生黃昂矩於多體學及精準醫學聯合會議壁報比賽榮獲第二名! Congratulations to Ang-Chu Huang (PhD candidate) on the Second Place Poster Award of Multiomics and Precision Medicine Joint Conference 
> 2023Sep
恭喜林倩伶老師獲得有庠基金會有庠科技論文獎! Y. Z. Hsu Science and Technology Paper Award Far Eastern Y. Z. Hsu Foundation, Taiwan 
>2023Aug
本實驗室獲頒國科會優秀年輕學者研究計畫! Outstanding Young Scholar Research Grant, National Science Council, Taiwan >2023Mar 恭喜GSB博班生黃昂矩榮獲RNA Society Research Presentation Fellowship! >2023Feb 恭喜江宏倫博士獲選為日本第14屆希望會議台灣代表! >2023Jan 賀!!! 恭喜TIGP博班生蘇家瑩榮獲TIGP學生傑出研究表現獎! Congratulations to Jia-Ying Su (PhD candidate) on winning the TIGP Research Performance Fellowship! > 2022Dec 賀!!! 恭喜江宏倫博士榮獲第32屆王民寧獎-優秀論文獎! 
> 2022Nov
恭喜GSB博班生黃昂矩於Nature Conference - RNA at the Bench and Bedside III (Carlsbad, CA, USA) 壁報展出獲得 Award for Excellence! 
> 2021Oct
恭喜江宏倫博士獲得台灣生化分生學會壁報論文佳作! > 2021 July
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> 2020 Aug
本實驗室獲頒科技部優秀年輕學者研究計畫!
Outstanding Young Scholar Research Grant, Ministry of Science and Technology, Taiwan
2020-2022
> 2020 Jan
本實驗室獲頒國家衛生研究院研究發展獎助!
Career Development Award, National Health Research Institutes, Taiwan 2020-2023
> 2019 Dec
恭喜TIGP博士候選人林靖茹獲得多體學及精準醫學聯合會議海報競賽第二名!
Ching-Ju Lin, Yun-Lin Wang and Chien-Ling Lin. Systematic Identification of Pathogenic Splicing Mutations of TSC1/2. 2019 Multiomics and Precision Medicine Joint Conference, Tainan, Taiwan. Dec 2019. (Second Place Poster Award)
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> 2018 July
恭喜Dr. YoonSoon Kang (姜允淳博士) 獲得中央研究院博士後研究學者獎助!
Congratulations to Dr. YoonSoon Kang on Postdoctoral Scholar Fellowship, Academia Sinica
our lab is supported by:
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